It is important to know the classification and selection of the syringe filter. The syringe filter is the main component of the syringe system for pollutant control. Filtering the sample solution is an important step in sample pretreatment. This filter is used for HPLC and IC analysis. Guidelines for the selection of syringe filters and filter membranes Selection of different types of aperture filters, membrane filters, and mobile phase samples for the clarification and removal of bacteria and impurities.
Selecting the right syringe filter is considered a key decision point in the analytical process of sample filtration and must be tested. The correct filter increases the accuracy of the experimental results. If the wrong filter is selected, impurities and unwanted particles are filtered out, which are easily extractable, and samples are tested incorrectly, resulting in inaccurate results and findings.
The nylon membrane syringe filter must be compatible with the chemical properties of the sample. The filter housing material should be compatible with the chemical properties of the samples. It must be highly resistant to HPLC solvents and chemicals. The nylon membrane is compatible with a wide range of aqueous and organic solvents.
Syringe filters consist of a membrane filter that contains a plastic housing made of polypropylene. Most types of syringe filters come with a selection of pore sizes. It is important to ensure that the pore size is suitable for your application to ensure that it works as you wish. Some of the most common pore sizes can be found in our series of examples for typical applications.
When selecting a syringe filter, several factors must be taken into account. Diaphragm diameter, material, pore size, and housing influence the right choice. Below, we look at the most important factors that will help you make the choice compatible. Sample volume: 10ml, 100ml, 250ml, 13mm, 25mm, 33mm, 4mm.
Cellulose acetate (CA): Cellulose acetate is a membrane material of hydrophilic nature used in biological and aqueous samples. It has little protein as a binder and is a strong membrane. It is the most commonly used hydrophobic filter membrane material in many laboratories for filtration.
Nylon membrane: The nylon filter membrane is a very strong hydrophilic membrane material used for filtration in aqueous solutions and serves as the basis for most HPLC solvents such as alcohols and aromatic hydrocarbons.
Hydrophilic syringe filters are the most popular choice for filtering aqueous solutions. Nylon, polyethersulfone, cellulose acetate syringe filters, just to name a few, filter water-based solutions due to their hydrophilic properties. Another option is to use hydrophobic syringes and filters that wet the filter membrane with alcohols to make it hydrophilic.
Mixed Cellulose Ester: MCE (Mixed Cellulose Ester) is an excellent choice for maximum protein recovery. It is a syringe filter with low protein binding, making it ideal for aqueous samples. Laboratory studies have shown that MCE binds fewer proteins than PVDF and PES in syringe filters.
There is a huge selection of syringe filters in all kinds of materials, pore sizes, and diameters. When it comes to choosing the right one for your application, it can seem like a minefield.
Syringe filters are disposable filter cartridges that are attached to the end of the syringe. They are used for universal filtration and are affordable, making them a great addition to any laboratory. It is mainly used to remove particles from your samples for high-performance liquid chromatography (HPLC).
The solution comes with a hydrophilic (PTFE) syringe filter such as Airekacell. As a natural hydrophobic PTFE, it is disadvantageous not to let aqueous solution pass, as many chemicals are diluted with water. However, it offers a selection of aqueous samples to dilute hard chemicals. It processes liquids at high temperatures with excellent flow rate, low protein binding and is compatible with most bases, acids, and organic solvents.
It has broad chemical compatibility with low protein binders and is a good choice for the high protein recovery that is required. It is regenerated cellulose and hydrophilic solvents are resistant to membranes.
General biological filtration, filtration of samples with high protein recovery. Do not use strong acids, bases, esters, ethers, ketones, etc. Do not use very strong acids such as chloroform (THF).
There are many different filter materials that can be used in syringe filters. The most common are cellulose acetate (CA) and PES filter, and PTFE membrane. Each material indicates variations in the effective filter area (EFA) and the capacity of the filter. The main differences are chemical compatibility, flow, and burst pressure. If you need to filter smaller particle sizes, for example, to remove colloids, other filter types are suitable, such as centrifugal filters.
If you wish to sterilize the aqueous solution with a sterile syringe, the filter is wrapped to ensure optimum performance. These are the options you can choose based on your sample procedure and application. If your aqueous solution goes through a filter sterilization process, an unsterile syringe filter will meet your needs.
The diameter of the syringe filter is a good indication of how well the EFA holds volume. By enlarging the filter or the EFA, contaminated samples can pass through the filter. Particle-laden liquid clogs the filter and cleans the liquid. When particles are removed from the liquid, they can clog the pores in the filter, reducing and blocking the usable part of the liquid. If the pressure required to push the liquid through a filter becomes too high, the filter block must be replaced.
These filters are available in various diameters, including 4mm, 13mm, 17mm, 20mm, 25mm, 30mm, and 33mm. For small volume samples such as 1 ml, a filter with 4mm diameter can be used, while for larger volumes such as 100 ml filters with 30mm diameter should be used.
In the laboratory, whether it is an inorganic experiment, an organic experiment, or a physical-chemical experiment, overkill operations are indispensable. Choosing a filter, especially a membrane filter, is a skill that every laboratory worker must master.
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